Antioxidant And Antibacterial Activity Of Methanolic Extract Of Machilus Odoratissima

Amit Subedi; Mohan Prasad Amatya; irtha Maiya Shrestha; Shyam Kumar Mishra; Bharat Mani Pokhrel

doi:10.70530/kuset.v8i1.319

Antioxidant And Antibacterial Activity Of Methanolic Extract Of Machilus Odoratissima

Authors

Amit Subedi Department of Pharmacy, Institute of Medicine, Tribhuvan University
Mohan Prasad Amatya Department of Plant Resources, Kathmandu
irtha Maiya Shrestha Department of Pharmacy, Kathmandu University
Shyam Kumar Mishra Department of Clinical Microbiology, Institute of Medicine, Tribhuvan University
Bharat Mani Pokhrel Department of Clinical Microbiology, Institute of Medicine, Tribhuvan University

DOI:

https://doi.org/10.70530/kuset.v8i1.319

Keywords:

Antibacterial, antioxidant, cup-plate, DPPH, lauraceae, Machilus odoratissima, Nepal

Abstract

The methanol extract of the bark of Machilus odoratissima was subjected to investigate its antioxidant and antibacterial properties. The phytochemical screening demonstrated the presence of different types of compound like terpenoids, tannins, deoxy sugar, saponins and phenolic compounds. The methanol extract of the plant was tested for antioxidant activity using scavenging activity of DPPH(1,1-diphenyl-2-picrylhydrazil) radical method and antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli & Pseudomonas aeruginosa bacteria using cup plate method. The extract exhibited high free radical scavenging activity. IC₅₀ was found to be 3.37μg/ml. Antibacterial activity was observed against S. aureus in dose dependent manner

Published

2012-03-16

How to Cite

Subedi, A., Amatya, M. P., Shrestha, irtha M., Mishra, S. K., & Pokhrel, B. M. (2012). Antioxidant And Antibacterial Activity Of Methanolic Extract Of Machilus Odoratissima. Kathmandu University Journal of Science Engineering and Technology, 8(1). https://doi.org/10.70530/kuset.v8i1.319