Effect of Different Sugars on Shoot Induction in CV. Basmati

Authors

  • Ruby Thapa Effect of various concentrations of different sugars was investigated for induction of root and shoot from basmati rice. Explant, seeds (grains) were transferred in ½ MS basal media fortified with different concentrations of carbon source like sucrose, maltose, and dextrose. In vitro regenerated rice plantlets were healthy and attained a length of 16.17cm at 0.25 % concentration within a week. Only low concentration (0.25%) of dextrose exhibited the maximum shoot and root growth, out of three sugars. Maltose showed the moderate growth response of shoot and root length in all the concentrations.
  • Dipesh Dhakal Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal
  • Dhurba Prasad Gauchan Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal

Keywords:

Sugar, Sucrose, Dextrose, Maltose, Rice, Tissue Culture

Abstract

Effect of various concentrations of different sugars was investigated for induction of root and shoot from basmati rice. Explant, seeds (grains) were transferred in ½ MS basal media fortified with different concentrations of carbon source like sucrose, maltose, and dextrose. In vitro regenerated rice plantlets were healthy and attained a length of 16.17cm at 0.25 % concentration within a week. Only low concentration (0.25%) of dextrose exhibited the maximum shoot and root growth, out of three sugars. Maltose showed the moderate growth response of shoot and root length in all the concentrations.

Published

2007-01-30

How to Cite

Thapa, R., Dhakal, D., & Gauchan, D. P. (2007). Effect of Different Sugars on Shoot Induction in CV. Basmati. Kathmandu University Journal of Science Engineering and Technology, 3(1). Retrieved from https://journals.ku.edu.np/kuset/article/view/204

Issue

Vol. 3 No. 1 (2007): Kathmandu University for Science, Engineering and Technology (KUSET)

Section

Original Research Articles
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